Analysis of Porcine DNA in Gelatine Products using SYBR Green Real Time Polymerase Chain Reaction for Halal Verification

Authors

  • Mohd Hazim Yusop Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Malaysia
  • Tean Pei Ling Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Malaysia
  • Moh Fadzelly Abu Bakar Faculty of Applied Science and Technology, Universiti Tun Hussein Onn Malaysia, Malaysia
  • Kamarul Rahim Kamarudin Faculty of Applied Science and Technology, Universiti Tun Hussein Onn Malaysia, Malaysia

DOI:

https://doi.org/10.31098/ihsatec.v15i1.593

Abstract

Real-time PCR was employed in this study to determine whether gelatine items from nearby marketplaces contained porcine DNA. The mitochondrial NADH dehydrogenase subunit 5 (MT-ND5) gene from pigs (Sus scrofa) was the subject of oligonucleotide primer design; the forward and the reverse primer sequences produced 161bp amplicon. SYBR Green fluorescent dye was used to detect swine DNA in samples of 22 different types of gelatine products, 12 of which had labels identifying their sources and indicating their certification as Halal, and the remaining 10 had neither labels nor certification information. The 40 cycles of positive amplication with a Ct value of 19.81 served as the criterion for determining the specificity of the primers. In this work, the detection threshold for porcine DNA is set at 0.001 µg/ml. The efficiency of the PCR in this study is 91.72%, and the R2 is 0.9985, which indicates good efficiency. The 12 labelled products exhibit positive amplifications for porcine gelatine products while exhibiting negative amplifications for both bovine and fish gelatine products in labelled samples. Nine out of the ten samples of gelatine products that were tested had positive amplifications without source or Halal labelling.

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Published

2022-12-14

How to Cite

Yusop, M. H., Ling, T. P., Bakar, M. F. A., & Kamarudin, K. R. (2022). Analysis of Porcine DNA in Gelatine Products using SYBR Green Real Time Polymerase Chain Reaction for Halal Verification. Proceedings of The International Halal Science and Technology Conference, 15(1), 39–47. https://doi.org/10.31098/ihsatec.v15i1.593