Formulation and Stability Determination of Anti-Acne Cream Containing Black Cumin Seed Oil and Kaolin Clay

Abstract

Acne is the most common skin problem that could occur to any individual. Nigella sativa seed oil and kaolin as natural antimicrobial agents have been utilized in anti-acne cream formulated in this study. This study aimed at the development of anti-acne cream with antimicrobial properties using a crude extract of black cumin (Nigella sativa L.). Anti-acne creams had been formulated from cream-based agents with various percentages of crude black cumin seed extract and 1.0% (w/w) of mineral clay (Kaolin). Physical properties and stability of anti-acne cream at various storage conditions, including incubation at and freeze-thaw at 4, 40, and 45 °C for 28 days. The results showed that developed anti-acne cream containing a crude black cumin seed extract in 0.1 and 1.5% (w/w) had good physical stability. Therefore, the suitable formulation was then tested for anti-Propionibacterium acnes (P. acnes) susceptibility by the broth dilution method. From these results, it was found that 1.0 % (w/w) of crude black cumin seed extract had the ability to inhibit P.acnes with MIC (minimal inhibition concentration) of 15.6 mg/mL.