Synergistic effect of Euphorbia Milii with Tannic Acid as a disinfectant against Escherichia coli and Staphylococcus aureus

Abstract

Herbal disinfectant is the cheapest and most unique way to clean a surface. This study focused on the synergistic impact of Euphorbia Milii and Tannic acid as a disinfectant against microorganisms. The aqueous solvent extract of plant leaves was used mixed with tannic acid against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria tested by the disk diffusion method. Both bacterial species were isolated from the kitchen surface. Minimum Inhibitory Concentration (MIC) was recorded with an optical density at 600 nm using a UV-spectrophotometer, which showed inhibition of bacterial growth in a cultural broth mixed with extract of Euphorbia Milii and Tannic acid. According to the findings, the disinfectant showed a maximum zone of inhibition for E. coli (14 mm) and S. aureus (20 mm). The disinfectant activities of extract were tested and estimated using a time-kill analysis. Fourier transform infrared spectroscopy (FTIR) analysis was conducted to identify the chemical bond, giving information related to the active sites of chemical compounds present in disinfectants. Overall, this study reveals that Euphorbia Milii is an excellent candidate to formulate disinfection.