Addition Of Metarhizium Anisopliae In Organic Fertilizer For Enhancing White Grub’s Control


  • Mofit Eko Poerwanto Universitas Pembangunan Nasional Veteran Yogyakarta
  • Chimayatus Solichah Universitas Pembangunan Nasional Veteran Yogyakarta
  • Danar Wicaksono Universitas Pembangunan Nasional Veteran Yogyakarta



M. anisopliae, compost fertilizer, white grubs, mortality, pathogenicity


White grub pests have been destroyed a lot of plantation of dry land. Farmers rely on synthetic insecticides to overcome the white grubs problem. Concerning the ecosystem pollution, it is necessary to improve the method of preparing M. anisopliae which is more effective on white grub-infested land to substitute synthetic insecticides. The research was conducted by mixing compost fertilizer produced by the Faculty of Agriculture UPN "Veteran" Yogyakarta with the M. anisopliae according to the treatment (6.25g / kg; 13.5g / kg; and 20.25g / kg). The pathogenicity test was carried out in a plastic container. Each container is filled with a compost mixture with compost and sand (1:10) and one white grub larvae instar 4. Each treatment consisted of 30 white grubs. The result reveals that mixing compost fertilizer in the application of M. anisopliae was effective in increasing white grubs mortality by up to 16.67%. The greater the dose of M. anisopliae will speed up the mortality time and increase the number of mortality.


Brühl., CA. & Zaller, JG. 2019. Biodiversity decline as a consequence of an inappropriate environmental risk assessment of pesticides

Edes, L., Järvan, M., Lauringson, E., Akk, E., Tamm, K., 2013. The effect of solid cattle manure on soil microbial activity and on plate count microorganisms in organic and conventional farming systems. Int. J. Curr. Microbiol. App. Sci 2: 476–488

Fuchslueger, L., Gentsch, N., Gittel, A., Guggenberger, G., Hofer, A., Kienzl, S., Knoltsch, A., Lashchinskiy, N., Mikutta, R., Hana, S., 2014. Effects of soil organic matter properties and microbial community composition on enzyme activities in cryoturbated arctic soils 9, Plos One. 10.1371/journal.pone.0094076.

Harjaka, T., Martono, E., Witjaksono, Sunarminto, BH. 2011. The potential of Metarhizium anisopliae to control white grubs that destroy sugar cane roots. Seminar Pesnab IV. Jakarta 15 Oktober 2011. P: 91-102 (in bahasa)

Haryadi, N. T., W. Jadmiko & S. Hasjim. 2013. Integration of the Application of Metarhizium anisopliae and Insect Pathogenic Nematodes as Biological Control Agents of Lepidiota Stigma larvae that Attack Sugarcane Plants. Fac. Of Agriculture Univ. Jember. (in Bahasa)

Indrayani, IGAA., Prabowo, H., Wijayanti, KS. 2019. The Effect of Addition of Manure and Chemical Insecticide Application on the Effectiveness of Metarhizium anisopliae on white grubs of Sugarcane, Lepidiota stigma. Bulletin Tanaman Tembakau, Serat & Minyak Industri. 11(1):33−45 (in Bahasa)

Lee, P.C & R.F. Hou. 2003. Pathogenesis of Metarhizium anisopliae var. anisopliae in the smaller brown planthopper Laodhelpax striatelus. J. Entomol.9: 13-19

Namasivayam, S.K.R., Aarthi, R., Anbazhahan, P., 2015. Studies on factors influencing the viability of entomopathogenic fungi Metarhizium anisopliae in soil adapting culture-dependent method. J. Biopestic. 8: 23–27.

Ojo, O.I., Olajire-Ajayi, B.L., Dada, O.V., Wahab, O.M, 2015. Effects of fertilizers on soil’s microbial growth and populations: a review. Am. J. Eng. Res. 4: 52–61.

Poerwanto, M.E. & D. Saidi. 2016. The ability of the fungus Metarhizium anisopliae in controlling urethra in peanut cultivation. Proceedings of the 2nd year National Seminar call for papers and an exhibition of the results of research & community service from the Ministry of Research, Technology and Higher Education of Indonesian economic governance in the ASEAN economic community and enhancing the dignity of the nation based on energy resources and strengthening research synergies between governments, industry & universities. UPN "Veteran" Yogyakarta

Poerwanto, M.E. & Mulyanto, D. 2017. The effectiveness of soil tillage in reducing the white grub population in peanut plantation. Techno, 3(1): 71-73

Poerwanto, ME., Solichah, C. 2010. Uretic behavior in compost mixture with tobacco plant waste. Proceedings of the 2010 national seminar on food & energy security. Fac. Of Agriculture UPN "Veteran" Yogyakarta